Tag Archives: PCR

Tech Mahindra For A Safe Global Reopening

To be sure of the safety and efficacy of Covid-19 screening technology that he was considering for his employees, Tech Mahindra CEO CP Gurnani, suggested testing it on himself and his household members. That decision may have saved him and his family from having to be admitted to hospital —as actors Amitabh and Abhishek Bachchan were last week. As it turned out, everyone in the house received a clean bill of health, except the two sons of his cook who were diagnosed as high risk.

As the Bachchans too must have done, Gurnani had had his domestic staff take all necessary precautions, remaining distant and wearing masks. His cook’s sons did not show any visible sign of infection, yet were potential Covid-19 carriers.

The fact is that the testing techniques in common use are inadequate, and social distancing isn’t always possible. RT-PCR (reverse transcription polymerase chain reaction) tests have ‘false negative’ rates of 20-67%, depending on when they are taken. Also, temperature screening and contact tracing fail to identify the presymptomatic spreaders who, according to mathematical modeling, could be responsible for half of the infections. When Delhi health minister Satyendar Jain showed symptoms, his first RT-PCR results came back negative. But they were positive the very next day.

The technology and techniques I had persuaded Gurnani and Tech Mahindra to pilot — and that I had a hand in developing — are based on understanding an individual’s risk and performing monthly testing. The reality is that even though Covid-19 can be devastating for a few, not everyone who gets infected will have serious symptoms. We can identify the people at high risk with reasonable accuracy based on studies from around the world and data from India. And we can give them special treatment.

For example, it is well established that men above 65, whose health is chronically compromised by diabetes, cardiovascular disease, or obesity, are at higher risk of severe outcomes. Further, severity can be predicted by a number of tests, including those for hypertension, diabetes, and obesity, and measures of lactate dehydrogenase (LDH) and D-dimer.

Businesses in the US have considered offering these types of tests. But the costs and logistics of doing so are usually prohibitive, with the most basic tests costing more than $100 apiece, and requiring the shipment of samples to labs. A single comprehensive screening for an individual could cost over $1,000, and is usually not covered by insurance plans. It can take days to get basic test results.

This is where India has an advantage over the West: it has developed screening devices, such as HealthCube, which can conduct a range of biochemical and physiological tests for a tiny fraction of the US cost. These include 12-lead electrocardiograms (ECG), tests for blood pressure, oxygen saturation, blood glucose, hemoglobin and cholesterol, and rapid diagnostic tests for infectious diseases.

With HealthCube, an entire regimen of tests, including a test for Covid-19 antibodies and severity markers, can be performed for less than Rs1,000 within minutes. The technology has received Europe’s CE certification.

The Covid-19 risk screening program underway at Tech Mahindra on the HealthCube platform uses patient risk factors — age, gender, medical conditions, potential exposure, recent travel or being in a crowded place, public health data, aggregations from previous screenings, patient symptoms, etc — to compute a risk score for patients. Those at high risk are checked for markers, such as D-dimer and troponin, which are elevated in those who develop the severe disease (which indicate heart ailments). These tests are followed up by teleconsultations and further testing, as appropriate.

With more data and testing, the artificial intelligence (AI) algorithms become increasingly accurate, and people are given an individualized screening and testing protocol based on their risk factors, rather than being treated like machines that need temperature checks.

Antibody tests have been controversial largely because the first generation of tests performed worldwide were mostly from China and were low quality and defective. There have also been doubts about whether all Covid-19 patients develop antibodies and, even if they do, how long the immunity lasts.

The newer generations of tests, made outside China, are highly accurate. Swiss pharma giant Roche, for example, claims that 14 days after infection, its test can detect antibodies with 99.8% specificity and 100% sensitivity. HealthCube claims that its India-made tests have 98% specificity and 96% sensitivity. These are both a huge leap from the 5.4% accuracy of the tests that China first sold to India.

There is substantial evidence that within 19 days after symptom onset, 100% of patients test positive for Covid-19 antibodies. And as Harvard epidemiologist Marc Lipsitch explained in the New York Times, ‘After being infected with SARS-CoV-2, most individuals will have an immune response, some better than others. That response, it may be assumed, will offer some protection over the medium term — at least a year — and then its effectiveness might decline.’ Even half a year will buy us time to understand and develop better approaches to prevention and treatment — and administer vaccines to those at high risk.

Gurnani told me that his personal experience, and the testing Tech Mahindra has conducted on a few hundred employees, have convinced him to offer the full health screening to the entire India Tech Mahindra employee base — including third-party employees, who typically can’t afford the test and are the most vulnerable because they stay in crowded places. He says that he puts his employees’ health above any business needs and cannot allow even one person to be at unnecessary risk.

Gurnani’s ambition is to show India — and the world — how to safely and sensibly open businesses and economies.

Vivek Wadhwa is a distinguished fellow and professor, Carnegie Mellon University’s College of Engineering, Silicon Valley.

This article was republished with permission from the author and can be originally found here.

Why Does COVID-19 Testing Take So Long?

After a slow start, testing for COVID-19 has ramped up in recent weeks, with giant commercial labs jumping into the effort, drive-up testing sites established in some places and new products approved under emergency rules set by the Food and Drug Administration.

But even for people who are able to get tested (and there’s still a big lag in testing ability in hot spots across the U.S.), there can be a frustratingly long wait for results — not just hours, but often days. Sen. Rand Paul (R-Ky.) didn’t get his positive test results for six days and is now being criticized for not self-quarantining during that time.

We asked experts to help explain why the turn-around time for results can vary widely — from hours to days or even a week — and how that might be changing.

It’s A Multistep Process

First, a sample is taken from a patient’s nose or throat, using a special swab. That swab goes into a tube and is sent to a lab. Some large hospitals have on-site molecular labs, but most samples are sent to outside labs for processing. More on that later.

That transit time usually runs about 24 hours, but it could be longer, depending on how far the hospital is from the processing lab.

Once at the lab, the specimen is processed, which means lab workers extract the virus’s RNA, the molecule that helps regulate genes.

“That step of cleaning, the RNA extraction step, is one limiting factor,” said Cathie Klapperich, vice chair of the department of biomedical engineering at Boston University. “Only the very biggest labs have automated ways of extracting RNA from a sample and doing it quickly.”

After the RNA is extracted, technicians also must carefully mix special chemicals with each sample and run those combinations in a machine for analysis, a process called polymerase chain reaction (PCR), which can detect whether the sample is positive or negative for COVID.

“Typically, a PCR test takes six hours from start to finish to complete,” said Kelly Wroblewski, director of infectious disease programs at the Association of Public Health Laboratories.

Some labs have larger staffs and more machines, so they can process more tests at a time than others. But even for those labs, as demand grows, so does the backlog.

Capacity Is Expanding, But Not Enough

Initially, only a few public health labs and the federal Centers for Disease Control and Prevention processed COVID-19 tests. Problems with the first CDC test kits also led to delays.

Now the CDC has a better kit, and 94 public health labs across the country do COVID-19 testing, said Wroblewski.

But those labs can’t possibly do all that’s needed. In normal times, their main function is regular public health surveillance — detecting more common threats such as outbreaks of measles or monitoring seasonal influenza — “but not to do diagnostic testing of the magnitude that is required in this response,” she said.

Large commercial labs like those run by companies such as Quest Diagnostics and LabCorp were given the go-ahead late last month by the FDA to start testing, too.

The FDA has said it won’t stop certain private labs — those that are already certified to perform complex testing — and diagnostic companies from developing their own test kits. Labs at some big-name hospital systems, such as Advent Health, the Cleveland Clinic and the University of Washington, are among those doing this.

In addition, the FDA has approved more than a dozen testing kits by various manufacturers or labs under special emergency rules designed to speed the process. Those include tests by Quest Diagnostics, LabCorp, Roche, Quidel Corp. and others. The kits are used in PCR machines, either in hospital labs or large commercial labs.

“Our chief medical officer on the East Coast said that, up until two days ago, on average it was taking 72 hours to get results,” said Susan Van Meter, executive director of AdvaMedDx, a division of the Advanced Medical Technology Association, a device and diagnostics industry trade group. “That will get better as our member companies come on the market.”

Even so, supply is not keeping up with demand, Roche CEO Severin Schwan told CNBC on Monday. Roche won the first approval from the FDA for a test kit under emergency rules, and it has delivered more than 400,000 kits so far.

“Demand continues to be much higher than supply,” Schwan told CNBC. “So we are glad that overall capacity is increasing, but the reality is that broad-based testing is not yet possible.”

How Many Tests Can Be Done At A Time?

That varies. Large commercial labs can do a lot. LabCorp, for example, said it is processing 20,000 tests a day — and hopes to do more soon. Other test kit makers and labs are also ramping up capacity.

Smaller labs — such as molecular labs at some hospitals — can do far fewer per day but get results to patients faster because they save on transit time.

Still, it’s usually only large academic medical centers and some health systems that have their own molecular labs, which require complex equipment.

One of those is Medstar Georgetown University Hospital in Washington, D.C.

“From beginning to results can take five to six hours,” said Joeffrey Chahine, technical director for the molecular pathology division there.

Even at such hospitals, the tests are often prioritized for patients who have been admitted and staff who might have been exposed to COVID-19, said Chahine. His lab can process 93 samples at a time and run a few cycles a day, up to about 280, he said. Last week, it did 186 a day, three days in a row.

But hospitals with this ability are generally “not testing from their outpatient centers or the ER,” he said. In other words, the in-house labs aren’t running tests from walk-in patients.

Those tests are sent to large outside labs “so as not to overwhelm the hospital lab.” While those outside labs have large staffs, “the demand is so high that these outpatient clinics and ERs say the turnaround time can be four to seven business days,” he said.

Supply Shortages Are Slowing Test Production

As the worldwide demand for testing has grown, so, too, have shortages of the chemical agents used in the test kits, the swabs used to get the samples, and the protective masks and gear used by health workers taking the samples.

“There is an inadequate supply of so many things associated with testing,” said Wroblewski, which is why her group, along with officials in states including New York and cities including Los Angeles, recommend prioritizing who should be tested for COVID-19.

At the front of the line, she said, should be health care workers and first responders; older adults who have symptoms, especially those living in nursing homes or assisted living residences; and people who may have other illnesses that would be treated differently if they were infected. Bottom line: prioritizing who is tested will help speed the turnaround time for getting results to people in these circumstances and reduce their risk of spreading the illness.

Still, urgent shortages of some of the chemicals needed to process the tests are hampering efforts to test health care workers, including at hospitals such as SUNY Downstate medical center in hard-hit New York.

Looking forward, companies are working on quicker tests. Indeed, the FDA in recent days has approved tests from two companies that promise results in 45 minutes or less. Those will be available only in hospitals that have special equipment to run them. One of those companies, Cepheid of Sunnyvale, California, says about 5,000 U.S. hospitals already have the equipment needed to process these tests. Both firms say they will ship to the hospitals soon but have given few specifics on quantity or timing.

But many public health officials say doctors and clinics need a truly rapid test they can use in their offices, one like the tests already in use for influenza or strep throat.

A number of companies are moving in that direction. Late Friday, for instance, Abbott Laboratories announced that the FDA has given emergency-use authorization for the company’s rapid, point-of-care test, which can deliver positive results in as little as five minutes and negative results in 13.

The tests are processed on a small device already installed in thousands of medical offices, ERs, urgent care clinics and other settings. Abbott said it will begin this week to make 50,000 tests available per day.

“That’s going to make a meaningful difference,” said Van Meter at AdvaMedDx, who believes the rapid tests are a critical piece in the continuum of available testing.

Even though lab-based PCR tests, which are done at large labs and academic medical centers, can take several hours to produce a result, the machines used can test high numbers of cases all at once. The rapid test by Abbott — and other, similar tests now under development — do far fewer at a time but deliver results much faster.

“This can be provided in a doctor’s office or an ER, helping to triage patients who are waiting to get in,” said Van Meter. “It’s a very fine complement to the testing that exists.”

This story was produced by Kaiser Health News, an editorially independent program of the Kaiser Family Foundation.</>